Research Article

Genetic diversity of the mangaba GeneBank using microsatellites

Published: February 26, 2019
Genet. Mol. Res. 18(1): GMR18108 DOI: https://doi.org/10.4238/gmr18108
Cite this Article:
A.V.C. Silva, M.F. Vitória, V.R.R. Azevedo, A.J. Sá, A.L.S. Nascimento, M.N. Cardoso, A.N.R. Soares, J.F.Silva Júnior1, A.S. Lédo (2019). Genetic diversity of the mangaba GeneBank using microsatellites. Genet. Mol. Res. 18(1): GMR18108. https://doi.org/10.4238/gmr18108
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Abstract

The mangaba, Hancornia speciosa, (Apocynaceae) is a fruit tree native to Brazil with predominantly extractivist production. The fruit can be consumed in natura; however, it is widely consumed as frozen pulp and ice cream produced by agro-industry companies. We evaluated the genetic diversity of 213 individuals that make up the mangaba GeneBank of Embrapa Tabuleiros Costeiros, using nine microsatellite markers (SSR). A total of 147 alleles were identified, with a mean of 16 alleles per locus; 100% polymorphism was observed among accessions. Reliability of the result was verified based on stress (0.042) and correlation (0.988) values. The alleles presented a high frequency of heterozygosity (He > Ho). The Fst (0.22) and f (0.07) values indicated moderate population structure, with great diversity within accessions. Bayesian analysis indicated the most adequate grouping with k = 2. The Unweighted Pair Group Method analysis showed three distinct groups according to similarity. The BI accession had the best genetic structure. The PM5/GX2, CN1/CN9, G18/PA1, JA14/JA15, and OI8/OI9 pairs of individuals are the closest genetically. We conclude that the Mangaba GeneBank has high diversity; this knowledge is relevant to develop strategies for the management of these genetic resources.

 

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