Research Article

Genetic diversity between and within half-sib families of Brazil nut tree (Bertholletia excelsa Bonpl.) originating from native forest of the Brazilian Amazon

Published: November 29, 2017
Genet. Mol. Res. 16(4): gmr16039839 DOI: 10.4238/gmr16039839

Abstract

Brazil nut tree is a species of economic importance for
the Amazon region, known for the commercialization of its almonds.
The objective of this work was to study the genetic diversity among halfsib
progenies from different Brazil nut trees present in native forest in
the municipality of Itaúba, MT, belonging to the Brazilian Amazon. In a
native forest area of nine hectares, fruits of nine parent trees, randomly
selected in the plot, were collected. The seeds were planted at
greenhouse and they were named, according to their origin, identifying
seed tree and fruit. After the seed germination and initial development
of the seedlings, leaves were collected for DNA extraction and analyzed
with microsatellite molecular markers. It was performed analysis of
molecular variance and cluster analysis of progenies and seed trees.
There is greater genetic diversity between families than among
progenies from the same family. The clustering of progenies from
different families in the same group can be explained by the low
dissimilarity between the seed trees. Among the loci analyzed in this
study, eight were informative for evaluations of genetic diversity in
Brazil nut, except BET12 and BET16 loci.
Download: