Research Article

Genetic divergence among Psidium accessions based on single nucleotide polymorphisms developed for Eucalyptus.

Published: May 04, 2017
Genet. Mol. Res. 16(2): gmr16029566 DOI: https://doi.org/10.4238/gmr16029566
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(2017). Genetic divergence among Psidium accessions based on single nucleotide polymorphisms developed for Eucalyptus.. Genet. Mol. Res. 16(2): gmr16029566. https://doi.org/10.4238/gmr16029566
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Abstract

The goal of this study was to analyze the genetic divergence among Psidium species accessions based on SNPs developed for Eucalyptus. Fifty-three Psidium accessions, including 47 P. guajava, were genotyped with EUCHIP60K. The dendrogram similarity ranged from 0.58 to 1.00, with a cophenetic value of 0.97. Five groups were identified at dendrogram cut point of 0.7: the first with 44 guava accessions, the second with 1 guava accession, the third with 3 P. guineense accessions, the forth with 2 guava accessions, and the fifth with 3 P. cattleianum accessions. The Bayesian analyses suggested seven subpopulations, with formation of two additional groups with guava accessions. Primers designed with Eucalyptus SNP sequences resulted in reliable Psidium amplicons on 6% polyacrylamide gels. In general, the SNP dendrogram agreed with biological genus structure, since different species were not grouped, indicating that transferability among Myrtaceae genus was possible and reliable.

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