Research Article

Evaluation of a cassava translationally controlled tumor protein (MeTCTP) reveals its function in thermotolerance of Escherichia coli and in vitro chaperone-like activity

Published: November 29, 2017
Genet. Mol. Res. 16(4): gmr16039835 DOI: https://doi.org/10.4238/gmr16039835
Cite this Article:
(2017). Evaluation of a cassava translationally controlled tumor protein (MeTCTP) reveals its function in thermotolerance of Escherichia coli and in vitro chaperone-like activity. Genet. Mol. Res. 16(4): gmr16039835. https://doi.org/10.4238/gmr16039835
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Abstract

The TCTP (Translationally controlled tumor protein)
is a highly conserved protein family found in eukaryotic organisms,
which has been associated to the performance of several functions at
the cellular level, as those related to growth, development, and
responses against environmental stresses. However, studies on TCTP
from plants are still incipient. Cassava (Manihot esculenta Crantz) is
a crop of great socioeconomic importance for millions of people in
the world, especially because of its high energy content. Previous
studies reported the identification of a cassava TCTP (MeTCTP)
with potential roles in storage root formation and salt stress response.
Here, our main goal was to increase the understanding about the roles
of MeTCTP in response to abiotic stresses. We verified that the
overexpression of recombinant MeTCTP in Escherichia coli
increased tolerance of bacterial cells to heat stress. In addition, the
recombinant MeTCTP was purified by nickel affinity and evaluated
by chaperone activity assays, which showed its ability to preserve the
function of NdeI restriction enzyme under thermal denaturation 
 
conditions. Results presented here provided insights into the
relationship of MeTCTP with responses of plants to abiotic stresses.
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