Research Article

Effect of You-Gui-Wan on endometrial angiogenesis in controlled ovarian hyperstimulation mice model

Published: November 29, 2017
Genet. Mol. Res. 16(4): gmr16039833 DOI: 10.4238/gmr16039833

Abstract

Purpose: To explore the mechanism of You-Gui-Wan
(YGW) in improving endometrial receptivity in mouse model of
controlled ovarian hyperstimulation (COH).
Methods: Sixty female mice with two consecutive oestrous cycles
were randomly divided into COH model group (n = 50) and normal
control group (n = 10). The COH model was created by using
GnRH-a + PMSG + HCG and further randomly divided into 5
subgroups of simple model, YGW high-dose, YGW medium-dose,
YGW low-dose and aspirin control, 10 in each subgroup. YGW or
aspirin or saline was administrated to the mice by gavage once a day
for 11 days. Afterwards, blood and uterine samples were collected
for examination of serum levels of estradiol (E2) and progesterone
(P4), endometrial thickness and number of blood vessels as well as
expressions of angiogenic factors of VEGF, Ang-1, Ang-2 and Tie2
at transcription and translation levels by using H& E staining,
ELISA, real-time qRT-PCR, and immunohistochemistry,
respectively.
Results: COH per se increased the serum concentrations of E2 and
P4,but decreased the number of endometrial blood vessels and the
protein expressions of the angiogenic factors when compared to the
normal control group (P< 0.05 or 0.01). YGW increased the mRNA
expressions of all angiogenic factors and restored the effect of COH
on number of endometrial blood vessels (P< 0.01), and the protein
expressions of the angiogenic factors when compared to the COH
simple model group (P< 0.05 or 0.01). In comparison, aspirin could
only restore the number of endometrial blood vessels and the protein
expressions of Ang-2 and Tie-2 (P< 0.05 or 0.01).
Conclusions: YGW can restore the side effect of COH on number
of endometrial blood vessels and the expressions of angiogenic
factors, thus improve endometrial receptivity in COH mice.
 
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