Research Article

The correlation analysis of polymorphisms of Prion-Related Doppel (PRND) with prion (PRNP) alleles in Gansu Alpine Merino sheep

Published: November 29, 2017
Genet. Mol. Res. 16(4): gmr16039832 DOI: https://doi.org/10.4238/gmr16039832
Cite this Article:
(2017). The correlation analysis of polymorphisms of Prion-Related Doppel (PRND) with prion (PRNP) alleles in Gansu Alpine Merino sheep. Genet. Mol. Res. 16(4): gmr16039832. https://doi.org/10.4238/gmr16039832
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Abstract

The interaction between the ovine prion protein gene
(PRNP) and Doppel (PRND) gene polymorphisms is essential for
understanding the role of prion proteins in scrapie. In this study, the
blood genomic DNA samples of 111 Gansu Alpine Merino sheep
were used to define the PRNP alleles and the haplotypes of PRND
by PCR-SSCP (single-strand conformation polymorphism) and
PCR. The results shown that the frequency of PRNP genotype
ARQ/ARQ and ARR/ARR has a certain advantage, especially the
ARQ/ARQ (46%). In addition, PRND 26 haplotypes and 29
polymorphisms sites were firstly defined, and the dominant
polymorphic sites are at codons 28, 51 and 158. The frequency of the
haplotype GTC of PRND is 98%, the ARQ/ARQ has great advantage 
in the PRNP genotype. These results may be helpful in future studies
of the relationship between PRND with PRNP in prion diseases. 
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