Research Article

Comparison of physical and chemical characteristics of collagen from the skin of cod (Gadus macrocephaius).

Published: June 29, 2017
Genet. Mol. Res. 16(2): gmr16027940 DOI: https://doi.org/10.4238/gmr16027940
Cite this Article:
(2017). Comparison of physical and chemical characteristics of collagen from the skin of cod (Gadus macrocephaius).. Genet. Mol. Res. 16(2): gmr16027940. https://doi.org/10.4238/gmr16027940
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Abstract

Acid and pepsin-soluble collagen (ASC and PSC, respectively) were extracted from cod (Gadus macrocephaius) skin, and yields of 37.36 and 55.96% were obtained for ASC and PSC, respectively. The total yield of ASC and PSC was 93.92%, based on the lyophilized dry weight, which is higher than that obtained from other sources. Electrophoresis revealed that both ASC and PSC consisted of two different α-chains (α1 and α2), which were characterized as type I collagen. Analysis of amino acids showed that both the ASC and PSC contained imino acids (216.1 and 190.6 residues/1000 residues, respectively) and the Fourier transform infrared spectroscopy spectra of both collagens were similar with pepsin hydrolysis having no effect on their triple-helical structure. The thermal denaturation temperature of ASC and PSC, as measured by viscometry, was 26.8° and 25.6°C, respectively.

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