Cloning and expression of class I chitinases in Hami melon after Penicillium infection.
Published: February 08, 2017Genet. Mol. Res. 16(1): gmr16019085 DOI: https://doi.org/10.4238/gmr16019085
Cite this Article:
F.Y. Jiang, B.B. Shui, F.X. Tang, C.H. Shan (2017). Cloning and expression of class I chitinases in Hami melon after Penicillium infection.. Genet. Mol. Res. 16(1): gmr16019085. https://doi.org/10.4238/gmr16019085
Chitinases are important disease-related proteins that play critical roles in plant defense against disease. To investigate the function of chitinases in the resistance of Hami melon to Penicillium infection, the gene encoding chitinases, HmCHT-2, was cloned and RT-PCR was used to measure expression levels of HmCHT-2. When the Hami melon was infected by Penicillium sp after 0, 12, 36, 48, 60, and 72 h. The results showed that comparing to the control group, the time of expression levels reaching to the peak delayed and the expression levels maintained at a significantly high level for a longer time. These results suggest that HmCHT-2 may contribute to the defense of Hami melon against fungal infection.