Research Article

CCR5Δ32 polymorphism analysis of lipodystrophy syndrome development in HIV/AIDS patients

Published: July 26, 2019
Genet. Mol. Res. 18(3): GMR18227 DOI: 10.4238/gmr18227

Abstract

The HIV/AIDS associated lipodystrophy syndrome is characterized by alterations in body fat and metabolism. We investigated whether CCR5Δ32 polymorphisms are associated with development of lipodystrophy. We made a case-control study of 248 HIV/AIDS patients who used antiretroviral therapy; they were divided into a group of patients (n = 148) who had lipodystrophy and a control group (n = 100) that did not present this syndrome. Both groups answered a questionnaire concerning age, gender and level of physical activity. DNA was extracted from blood samples using the mini salting out technique and PCR to identify polymorphisms of the CCR5Δ32 gene. Among lipodystrophy patients, most were men (64.8%) and 56.2% were sedentary. We found no significant association of the CCR5Δ32 allele with prevalence of lipodystrophy. However, male homozygotes were more susceptible to lipodystrophy (OR 1.78, P = 0.0350). We observed that the highest incidence of lipodystrophy was in sedentary men. More studies would be needed, using other markers, to allow precise use of genetic polymorphisms as a diagnostic parameter for lipodystrophy syndrome.

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