Research Article

Isolation and characterization of microsatellite loci in Quercus fabri (Fagaceae)

Published: June 21, 2016
Genet. Mol. Res. 15(2): gmr8322 DOI: https://doi.org/10.4238/gmr.15028322
Cite this Article:
Z.Z. Xiao, W.W. Chen, W. Bao, R. Wang, Y.Y. Li, Z.Z. Xiao, W.W. Chen, W. Bao, R. Wang, Y.Y. Li (2016). Isolation and characterization of microsatellite loci in Quercus fabri (Fagaceae). Genet. Mol. Res. 15(2): gmr8322. https://doi.org/10.4238/gmr.15028322
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Abstract

Quercus fabri is a pioneer species of secondary succession in evergreen broadleaved forests in China. In this study, we isolated and developed 12 polymorphic and 2 monomorphic microsatellite loci for Q. fabri using the biotin-streptavidin capture method. We characterized 12 polymorphic loci in 52 individuals from two populations. The number of alleles per locus ranged from 3 to 23. The observed and expected heterozygosities per locus were 0.033-0.773 and 0.138-0.924, respectively. These microsatellite loci will facilitate the studies on genetic variation, mating system, and gene flow of Q. fabri.

Quercus fabri is a pioneer species of secondary succession in evergreen broadleaved forests in China. In this study, we isolated and developed 12 polymorphic and 2 monomorphic microsatellite loci for Q. fabri using the biotin-streptavidin capture method. We characterized 12 polymorphic loci in 52 individuals from two populations. The number of alleles per locus ranged from 3 to 23. The observed and expected heterozygosities per locus were 0.033-0.773 and 0.138-0.924, respectively. These microsatellite loci will facilitate the studies on genetic variation, mating system, and gene flow of Q. fabri.