Research Article

STAT1 inhibitor alleviates spinal cord injury by decreasing apoptosis

Published: March 28, 2016
Genet. Mol. Res. 15(1): gmr7271 DOI: 10.4238/gmr.15017271

Abstract

Spinal cord injury (SCI) is typically caused by trauma or disease, and it severely affects patients’ motor function. The relationship between signal transducers and activators of transcription-1 (STAT1) and neuronal death after cerebral focal ischemia has been comprehensively studied, but its role in SCI remains largely unknown. This study investigated the protective effect of an STAT1 inhibitor on SCI. Thirty SD rats were SCI-induced and were then randomly divided into two groups (N = 15 each), either receiving STAT1 or the STAT1 inhibitor S1491 by intraperitoneal injection. The motor dysfunction of the rats was evaluated by behavioral scores, followed by the examination of SCI by hematoxylin and eosin staining. Apoptosis was also detected by Western blot and terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labeling (TUNEL) assay. The motor functions of rats receiving STAT1 did not score as well as the STAT1 inhibitor group (P

Spinal cord injury (SCI) is typically caused by trauma or disease, and it severely affects patients’ motor function. The relationship between signal transducers and activators of transcription-1 (STAT1) and neuronal death after cerebral focal ischemia has been comprehensively studied, but its role in SCI remains largely unknown. This study investigated the protective effect of an STAT1 inhibitor on SCI. Thirty SD rats were SCI-induced and were then randomly divided into two groups (N = 15 each), either receiving STAT1 or the STAT1 inhibitor S1491 by intraperitoneal injection. The motor dysfunction of the rats was evaluated by behavioral scores, followed by the examination of SCI by hematoxylin and eosin staining. Apoptosis was also detected by Western blot and terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labeling (TUNEL) assay. The motor functions of rats receiving STAT1 did not score as well as the STAT1 inhibitor group (P