Research Article

Estrogenic effects of flavonoid components in Xiaoyao powder

Published: February 11, 2016
Genet. Mol. Res. 15(1): gmr7500 DOI: 10.4238/gmr.15017500

Abstract

The objective of this study was to evaluate the estrogenic effects and mechanisms of three flavonoid components in Xiaoyao powder: quercetin, kaempferol, and isorhamnetin. The drugs were used to treat estrogen receptor (ER)-positive human breast cancer MCF-7 cells, and proliferation was measured using the MTT method. The expression of proteins and mRNA of the ER subtype were measured using western blotting and real time polymerase chain reaction. The quercetin (10-2 μM, 10-3 μM), kaempferol (100 μM, 10-2 μM), and isorhamnetin (10-3 μM) promoted the proliferation of MCF-7 cells, and the expression of ERα and ERβ proteins and mRNA were all increased significantly (P

The objective of this study was to evaluate the estrogenic effects and mechanisms of three flavonoid components in Xiaoyao powder: quercetin, kaempferol, and isorhamnetin. The drugs were used to treat estrogen receptor (ER)-positive human breast cancer MCF-7 cells, and proliferation was measured using the MTT method. The expression of proteins and mRNA of the ER subtype were measured using western blotting and real time polymerase chain reaction. The quercetin (10-2 μM, 10-3 μM), kaempferol (100 μM, 10-2 μM), and isorhamnetin (10-3 μM) promoted the proliferation of MCF-7 cells, and the expression of ERα and ERβ proteins and mRNA were all increased significantly (P