Research Article

Association between diabetes type 1 and DQB1* alleles in a case-control study conducted in Montevideo, Uruguay

Published: February 06, 2003
Genet. Mol. Res. 2 (1) : 29-35
Cite this Article:
A. Mimbacas, F. Pérez-Bravo, P.C. Hidalgo, G. Javiel, C. Pisciottano, R. Grignola, A.María Jorge, J.Pablo Gallino, J. Gasagoite, H. Cardoso (2003). Association between diabetes type 1 and DQB1* alleles in a case-control study conducted in Montevideo, Uruguay. Genet. Mol. Res. 2(1): 29-35.
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Abstract

We studied HLA DQB1 allele frequencies and the relative risk (RR) of various genotypes in 72 type 1 diabetic patients and 40 control individuals in Uruguay. This is a tri-racial (Caucasian, Black and Indo-American) mixed population. The products of the polymerase chain reaction amplifications were hybridized with oligonucleotides by allele-specific oligonucleotide reverse or dot blot methods. Significant differences between these two groups were observed only for allele DQB1*0302 (35%, RR = 7.34, P<0.001). The frequency of the alleles carrying a non-aspartic acid residue at position 57 was significantly higher in the diabetic patients (85 vs 53%, P<0.001). In contrast, the frequency of Asp alleles was negatively associated with type 1 diabetes (RR = 0.20, P<0.001). The genotype DQB1*0302/DQB1*0201 (33%, RR = 5.41, P<0.05) was positively associated with this disease. The genotype frequencies associated with type 1 diabetes in our population were significantly different from what is known for Caucasian and Black populations as well as compared with another admixed population, from Chile.

We studied HLA DQB1 allele frequencies and the relative risk (RR) of various genotypes in 72 type 1 diabetic patients and 40 control individuals in Uruguay. This is a tri-racial (Caucasian, Black and Indo-American) mixed population. The products of the polymerase chain reaction amplifications were hybridized with oligonucleotides by allele-specific oligonucleotide reverse or dot blot methods. Significant differences between these two groups were observed only for allele DQB1*0302 (35%, RR = 7.34, Pvs 53%, P

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