Research Article

Association of a hypoxia-inducible factor-3α gene polymorphism with superovulation traits in Changbaishan black cattle

Published: November 19, 2015
Genet. Mol. Res. 14 (4) : 14539-14547 DOI: 10.4238/2015.November.18.17

Abstract

This study was designed to examine a single nucleotide polymorphism (SNP) in the HIF-3α gene in three hundred Changbaishan black cattle using PCR-restriction fragment length polymorphism to determine whether there is an association between this SNP and superovulation. The cloning and sequencing results indicate that the polymorphism is due to a point mutation at the 278-bp position in the HIF-3α gene, resulting in 3 genotypes (AA, AB, and BB). Association analysis indicated that the polymorphism has a significant effect on the number of unfertilized embryos (NUE) (P HIF-3α could serve as a useful biomarker for donor selection, superovulation improvement, and assisted fertility.

This study was designed to examine a single nucleotide polymorphism (SNP) in the HIF-3α gene in three hundred Changbaishan black cattle using PCR-restriction fragment length polymorphism to determine whether there is an association between this SNP and superovulation. The cloning and sequencing results indicate that the polymorphism is due to a point mutation at the 278-bp position in the HIF-3α gene, resulting in 3 genotypes (AA, AB, and BB). Association analysis indicated that the polymorphism has a significant effect on the number of unfertilized embryos (NUE) (P HIF-3α could serve as a useful biomarker for donor selection, superovulation improvement, and assisted fertility.