Research Article

Isolation and characterization of novel polymorphic microsatellite markers for Lutjanus erythropterus

Published: September 21, 2015
Genet. Mol. Res. 14 (3) : 10944-10947 DOI: 10.4238/2015.September.21.5

Abstract

We isolated and characterized 22 polymorphic microsatellite loci in the crimson snapper (Lutjanus erythropterus) using a (GT)13-enriched genomic library. We found three to 15 alleles per locus, with a mean of 6.68. The observed and expected heterozygosities ranged from 0.087 to 0.978 and from 0.125 to 0.904, respectively, with averages of 0.576 and 0.650, respectively. Only three loci showed significant deviation from the Hardy-Weinberg equilibrium after Bonferroni correction. Four loci showed evidence for null alleles. These markers will be useful for analyzing the population genetic structure and gene flow of L. erythropterus.

We isolated and characterized 22 polymorphic microsatellite loci in the crimson snapper (Lutjanus erythropterus) using a (GT)13-enriched genomic library. We found three to 15 alleles per locus, with a mean of 6.68. The observed and expected heterozygosities ranged from 0.087 to 0.978 and from 0.125 to 0.904, respectively, with averages of 0.576 and 0.650, respectively. Only three loci showed significant deviation from the Hardy-Weinberg equilibrium after Bonferroni correction. Four loci showed evidence for null alleles. These markers will be useful for analyzing the population genetic structure and gene flow of L. erythropterus.

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