Research Article

Mendelian inheritance, genetic linkage, and genotypic disequilibrium at microsatellite loci in Genipa americana L. (Rubiaceae)

Published: July 27, 2015
Genet. Mol. Res. 14 (3) : 8161-8169 DOI: https://doi.org/10.4238/2015.July.27.4
Cite this Article:
(2015). Mendelian inheritance, genetic linkage, and genotypic disequilibrium at microsatellite loci in Genipa americana L. (Rubiaceae). Genet. Mol. Res. 14(3): gmr5129. https://doi.org/10.4238/2015.July.27.4
1,235 views

Abstract

Genipa americana is a tropical tree species that is widely distributed in the humid tropical and subtropical regions of Central and South America. This study investigated Mendelian inheritance, ge­netic linkage, and genotypic disequilibrium at six microsatellite loci developed for G. americana. Adult trees (188) and regenerants (163) were sampled and genotyped in a fragmented population of the spe­cies. We also genotyped open-pollinated seeds from 12 seed-trees dur­ing reproductive events in 2010 and 2011. Significant deviations from the expected 1:1 Mendelian segregation were detected in 29.5% of the tests. Significant genetic linkage between pairwise loci was detected in 54.4% of the tests, but no genotypic disequilibrium was detected between pairwise loci for adult trees and regenerants. Overall, the re­sults indicate that the six loci analyzed may be used in studies of G. americana’s genetic diversity and structure, its mating system, and in parentage analyses.

Genipa americana is a tropical tree species that is widely distributed in the humid tropical and subtropical regions of Central and South America. This study investigated Mendelian inheritance, ge­netic linkage, and genotypic disequilibrium at six microsatellite loci developed for G. americana. Adult trees (188) and regenerants (163) were sampled and genotyped in a fragmented population of the spe­cies. We also genotyped open-pollinated seeds from 12 seed-trees dur­ing reproductive events in 2010 and 2011. Significant deviations from the expected 1:1 Mendelian segregation were detected in 29.5% of the tests. Significant genetic linkage between pairwise loci was detected in 54.4% of the tests, but no genotypic disequilibrium was detected between pairwise loci for adult trees and regenerants. Overall, the re­sults indicate that the six loci analyzed may be used in studies of G. americana’s genetic diversity and structure, its mating system, and in parentage analyses.