Research Article

rbcS SRS4 promoter from Glycine max and its expression activity in transgenic tobacco

Published: July 03, 2015
Genet. Mol. Res. 14 (3) : 7395-7405 DOI: 10.4238/2015.July.3.15

Abstract

The regulatory region of the ribulose-1,5-bisphos­phate carboxylase small subunit gene SRS4 from soybean (Glycine max) was cloned using TAIL-PCR and general PCR, and named the rbcS promoter. The promoter was fused with the GUS gene and in­troduced into Nicotiana tabacum via Agrobacterium-mediated leaf disk transformation. In 4-week-old transgenic tobacco plants, the highest GUS expression levels were observed in the leaves, GUS activity was 7.13- and 7.40-fold higher in leaves than in stems and roots, respectively. Moreover, GUS activity was stimulated by light. In conclusion, spatial and light regulation of the soybean rbcS pro­moter was observed in N. tabacum, thus illustrating a leaf-specific and light-induced promoter.

The regulatory region of the ribulose-1,5-bisphos­phate carboxylase small subunit gene SRS4 from soybean (Glycine max) was cloned using TAIL-PCR and general PCR, and named the rbcS promoter. The promoter was fused with the GUS gene and in­troduced into Nicotiana tabacum via Agrobacterium-mediated leaf disk transformation. In 4-week-old transgenic tobacco plants, the highest GUS expression levels were observed in the leaves, GUS activity was 7.13- and 7.40-fold higher in leaves than in stems and roots, respectively. Moreover, GUS activity was stimulated by light. In conclusion, spatial and light regulation of the soybean rbcS pro­moter was observed in N. tabacum, thus illustrating a leaf-specific and light-induced promoter.