Research Article

Impact of BMMSCs from different sources on proliferation of CD34+ cells

Published: January 23, 2015
Genet. Mol. Res. 14 (1) : 474-482 DOI: 10.4238/2015.January.23.22

Abstract

There are significant differences on the biological characteristics of bone marrow mesenchymal stem cells (BMMSCs), immunological response, and antigen-presenting functions between patients with psoriasis and normal subjects, but there are no significant differences in aborted fetuses. We examined the differences in BMMSCs between aborted fetuses and patients with psoriasis in this study. Bone marrow from normal subjects, aborted fetuses, and patients with psoriasis were obtained using a MidiMACS machine. Density gradient centrifugation method was used to isolate the bone marrow mononuclear cells of patients with psoriasis and aborted fetus and the cells were cultivated. Bone marrow CD34+ cells from normal subjects were isolated. MTT colorimetric detection was used to test the proliferation activity of bone marrow CD34+ cells. The purity of bone marrow CD34+ cells and BMMSCs was determined by flow cytometry. The BMMSC culture supernatant fluid of patients with psoriasis and aborted fetuses showed no statistically significant difference with bone marrow CD34+ cell proliferation in normal subjects (P > 0.05).

There are significant differences on the biological characteristics of bone marrow mesenchymal stem cells (BMMSCs), immunological response, and antigen-presenting functions between patients with psoriasis and normal subjects, but there are no significant differences in aborted fetuses. We examined the differences in BMMSCs between aborted fetuses and patients with psoriasis in this study. Bone marrow from normal subjects, aborted fetuses, and patients with psoriasis were obtained using a MidiMACS machine. Density gradient centrifugation method was used to isolate the bone marrow mononuclear cells of patients with psoriasis and aborted fetus and the cells were cultivated. Bone marrow CD34+ cells from normal subjects were isolated. MTT colorimetric detection was used to test the proliferation activity of bone marrow CD34+ cells. The purity of bone marrow CD34+ cells and BMMSCs was determined by flow cytometry. The BMMSC culture supernatant fluid of patients with psoriasis and aborted fetuses showed no statistically significant difference with bone marrow CD34+ cell proliferation in normal subjects (P > 0.05).