Research Article

Isolation and characterization of microsatellite markers for Amomum tsaoko (Zingiberaceae), an economically important plant in China

Published: October 08, 2014
Genet. Mol. Res. 13 (4) : 8220-8224 DOI: https://doi.org/10.4238/2014.October.8.3
Cite this Article:
(2014). Isolation and characterization of microsatellite markers for Amomum tsaoko (Zingiberaceae), an economically important plant in China. Genet. Mol. Res. 13(4): gmr3908. https://doi.org/10.4238/2014.October.8.3
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Abstract

Twenty-four microsatellite markers were isolated from the genomic DNA of Amomum tsaoko Crevost et Lemaire, an important economic plant in China, using the method fast isolation by AFLP of sequences containing repeats (FIASCO). Polymorphism within each locus was assessed in 60 individuals from three populations in Yunnan Province, China, and nine of them were polymorphic. The number of alleles per polymorphic locus was 2, and the expected and observed heterozygosities ranged from 0.224 to 0.513, and from 0.050 to 0.600, respectively. Among nine microsatellite markers with polymorphism, five showed significant deviation from Hardy-Weinberg equilibrium (P

Twenty-four microsatellite markers were isolated from the genomic DNA of Amomum tsaoko Crevost et Lemaire, an important economic plant in China, using the method fast isolation by AFLP of sequences containing repeats (FIASCO). Polymorphism within each locus was assessed in 60 individuals from three populations in Yunnan Province, China, and nine of them were polymorphic. The number of alleles per polymorphic locus was 2, and the expected and observed heterozygosities ranged from 0.224 to 0.513, and from 0.050 to 0.600, respectively. Among nine microsatellite markers with polymorphism, five showed significant deviation from Hardy-Weinberg equilibrium (P