Research Article

RNA interference of leptin receptor in chicken adipocytes

Published: August 07, 2014
Genet. Mol. Res. 13 (3) : 5901-5907 DOI: https://doi.org/10.4238/2014.August.7.5
Cite this Article:
A.X. Huang, J.J. Li, Y. Tian, J.D. Shen, Z.R. Tao, G.Q. Li, L.Z. Lu, Y. Fu, T.X. Wu (2014). RNA interference of leptin receptor in chicken adipocytes. Genet. Mol. Res. 13(3): 5901-5907. https://doi.org/10.4238/2014.August.7.5
3,700 views

Abstract

In this study, chicken adipocytes were cultured to evaluate RNA interference by the leptin receptor gene. A small interfering RNA of the leptin receptor gene was synthesized, with a suppression rate of 60% being generated (P < 0.01). After the knockdown of the leptin receptor, the expression levels of certain genes decreased significantly; specifically, peroxisome proliferator-activated receptor γ, fatty acid synthase, adipose triglyceride lipase, and lipoprotein lipase. In addition, a significant increase in the expression of the adiponectin gene was documented. These results demonstrate that the leptin receptor gene might contribute to lipid metabolism by influencing the expressions of the peroxisome proliferator-activated receptor γ, fatty acid synthase, adipose triglyceride lipase, lipoprotein lipase, and adiponectin genes.

In this study, chicken adipocytes were cultured to evaluate RNA interference by the leptin receptor gene. A small interfering RNA of the leptin receptor gene was synthesized, with a suppression rate of 60% being generated (P peroxisome proliferator-activated receptor γ, fatty acid synthase, adipose triglyceride lipase, and lipoprotein lipase. In addition, a significant increase in the expression of the adiponectin gene was documented. These results demonstrate that the leptin receptor gene might contribute to lipid metabolism by influencing the expressions of the peroxisome proliferator-activated receptor γ, fatty acid synthase, adipose triglyceride lipase, lipoprotein lipase, and adiponectin genes.