Short Communication

Microsatellite markers derived from Japanese scallop (Mizuhopecten yessoensis) expressed sequence tags

Published: March 24, 2014
Genet. Mol. Res. 13 (1) : 1989-1992 DOI: 10.4238/2014.March.24.3

Abstract

Japanese scallop (Mizuhopecten yessoensis) is a cold-water shellfish, and a species of economic importance in China. In this study, we developed and evaluated simple sequence repeat (SSR) markers from the expressed sequence tags (ESTs) of M. yessoensis. The characteristics of 12 EST-SSR loci were investigated in 30 individual scallops, and the result revealed that the number of alleles per locus ranged from 2-4, with an observed heterozygosity ranging from 0.0333-0.7692, and an expected heterozygosity ranging from 0.0333-0.6312. Only two loci were found to depart significantly from the Hardy-Weinberg equilibrium (P M. yessoensis and its intraspecific variation.

Japanese scallop (Mizuhopecten yessoensis) is a cold-water shellfish, and a species of economic importance in China. In this study, we developed and evaluated simple sequence repeat (SSR) markers from the expressed sequence tags (ESTs) of M. yessoensis. The characteristics of 12 EST-SSR loci were investigated in 30 individual scallops, and the result revealed that the number of alleles per locus ranged from 2-4, with an observed heterozygosity ranging from 0.0333-0.7692, and an expected heterozygosity ranging from 0.0333-0.6312. Only two loci were found to depart significantly from the Hardy-Weinberg equilibrium (P M. yessoensis and its intraspecific variation.