Research Article

Extraction of total DNA and optimization of the RAPD reaction system in Dioscorea opposita Thunb.

Published: February 28, 2014
Genet. Mol. Res. 13 (1) : 1339-1347 DOI: https://doi.org/10.4238/2014.February.28.6
Cite this Article:
(2014). Extraction of total DNA and optimization of the RAPD reaction system in Dioscorea opposita Thunb.. Genet. Mol. Res. 13(1): gmr2964. https://doi.org/10.4238/2014.February.28.6
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Abstract

Dioscorea opposita Thunb. has been used as health food and herbal medicinal ingredients in traditional Chinese medicine. In this study, the total DNA of D. opposita Thunb. was extracted using an improved cetyltrimethylammonium bromide (CTAB) method, and the extracted DNA was further used for random amplified polymorphic DNA (RAPD) reaction system by design of the L16 (44) orthogonal diagram. The results showed that the improved CTAB method can be used to isolate high-quality and high-concentration DNA, and the optimized protocol can overcome the instability of RAPD reaction system. The knowledge stated here can be used to study the genetic diversity of D. opposita Thunb.

Dioscorea opposita Thunb. has been used as health food and herbal medicinal ingredients in traditional Chinese medicine. In this study, the total DNA of D. opposita Thunb. was extracted using an improved cetyltrimethylammonium bromide (CTAB) method, and the extracted DNA was further used for random amplified polymorphic DNA (RAPD) reaction system by design of the L16 (44) orthogonal diagram. The results showed that the improved CTAB method can be used to isolate high-quality and high-concentration DNA, and the optimized protocol can overcome the instability of RAPD reaction system. The knowledge stated here can be used to study the genetic diversity of D. opposita Thunb.