Research Article

Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis of Escherichia coli categories

Published: January 29, 2014
Genet. Mol. Res. 13 (1) : 716-722 DOI: https://doi.org/10.4238/2014.January.29.2
Cite this Article:
(2014). Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis of Escherichia coli categories. Genet. Mol. Res. 13(1): gmr2752. https://doi.org/10.4238/2014.January.29.2
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Abstract

The mass profiles of cell-free extracts of 180 commensal and pathogenic strains of Escherichia coli were determined by MALDI-TOF mass spectrometry (MS). While some peaks were highly conserved in all E. coli, several peaks occurred only in some strains, showing heterogeneity among them. We did not detect strain-specific peaks for any of the E. coli categories tested. However, review of the fully conserved and the variable peaks suggested that MALDI-TOF MS has the potential to distinguish commensal and uropathogenic E. coli strains. Additionally, eight Shigella sonnei isolates were tested and found to be indistinguishable from E. coli by MALDI-TOF MS under the test conditions.

The mass profiles of cell-free extracts of 180 commensal and pathogenic strains of Escherichia coli were determined by MALDI-TOF mass spectrometry (MS). While some peaks were highly conserved in all E. coli, several peaks occurred only in some strains, showing heterogeneity among them. We did not detect strain-specific peaks for any of the E. coli categories tested. However, review of the fully conserved and the variable peaks suggested that MALDI-TOF MS has the potential to distinguish commensal and uropathogenic E. coli strains. Additionally, eight Shigella sonnei isolates were tested and found to be indistinguishable from E. coli by MALDI-TOF MS under the test conditions.