Research Article

An effective method for extracting total RNA from Dioscorea opposita Thunb.

Published: January 21, 2014
Genet. Mol. Res. 13 (1) : 462-468 DOI: https://doi.org/10.4238/2014.January.21.15
Cite this Article:
(2014). An effective method for extracting total RNA from Dioscorea opposita Thunb.. Genet. Mol. Res. 13(1): gmr2953. https://doi.org/10.4238/2014.January.21.15
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Abstract

Dioscorea opposita Thunb., included in the genus Dioscorea of the family Dioscoreaceae, is an important herb with great edible and medicinal value. In this study, the total RNA from leaves of Lichuan Dioscorea opposita Thunb. was isolated by an improved Trizol method. The results showed that the RNA extracted by the improved Trizol method had good integrity, and the RNA could be used for down-stream molecular biology operations including reverse transcription-polymerase chain reaction.

Dioscorea opposita Thunb., included in the genus Dioscorea of the family Dioscoreaceae, is an important herb with great edible and medicinal value. In this study, the total RNA from leaves of Lichuan Dioscorea opposita Thunb. was isolated by an improved Trizol method. The results showed that the RNA extracted by the improved Trizol method had good integrity, and the RNA could be used for down-stream molecular biology operations including reverse transcription-polymerase chain reaction.

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