Research Article

Changes in peripheral blood Th1 and Th2 cells in rat liver transplantation under different immune statuses

Published: December 19, 2013
Genet. Mol. Res. 12 (4) : 6939-6946 DOI: 10.4238/2013.December.19.13

Abstract

In this study, early expressions of peripheral blood Th1 and Th2 cells were documented following rat liver transplantation and related to immune status. Rats were divided into 3 groups: group A (control): syngeneic transplantation (Brown Norway (BN) → BN); group B: allogeneic transplantation + cyclosporine A (CsA); group C: allogeneic transplantation (Lewis → BN). Flow cytometry was used to analyze peripheral blood CD4+CD45RC percentage on days 1, 3, 5, 7, and 14 following transplantation, and were compared to graft rejection pathological grades and receptor survival times. The average survival of groups A and B exceeded 100 days, which was significantly longer than that of group C (3.56 ± 34.3 days). With the exception of the first day, rejection grades were significantly higher in groups C and B compared to group A, and group C rejection grades were significantly higher than those of group B. Three days after transplantation, the CD4+CD45RC+ to CD4+CD45RC- ratio of group C was significantly higher than that of groups A and B. In group B, the CD4+CD45RC+ to CD4+CD45RC- ratio was negatively correlated to the rejection grade (r = -0.565, P

In this study, early expressions of peripheral blood Th1 and Th2 cells were documented following rat liver transplantation and related to immune status. Rats were divided into 3 groups: group A (control): syngeneic transplantation (Brown Norway (BN) → BN); group B: allogeneic transplantation + cyclosporine A (CsA); group C: allogeneic transplantation (Lewis → BN). Flow cytometry was used to analyze peripheral blood CD4+CD45RC percentage on days 1, 3, 5, 7, and 14 following transplantation, and were compared to graft rejection pathological grades and receptor survival times. The average survival of groups A and B exceeded 100 days, which was significantly longer than that of group C (3.56 ± 34.3 days). With the exception of the first day, rejection grades were significantly higher in groups C and B compared to group A, and group C rejection grades were significantly higher than those of group B. Three days after transplantation, the CD4+CD45RC+ to CD4+CD45RC- ratio of group C was significantly higher than that of groups A and B. In group B, the CD4+CD45RC+ to CD4+CD45RC- ratio was negatively correlated to the rejection grade (r = -0.565, P