Research Article

A generic plant RNA isolation method suitable for RNA-Seq and suppression subtractive hybridization

Published: November 18, 2013
Genet. Mol. Res. 12 (4) : 5537-5546 DOI: https://doi.org/10.4238/2013.November.18.4
Cite this Article:
(2013). A generic plant RNA isolation method suitable for RNA-Seq and suppression subtractive hybridization. Genet. Mol. Res. 12(4): gmr2483. https://doi.org/10.4238/2013.November.18.4
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Abstract

A recently developed revolutionary approach to transcrip­tomics, RNA-Seq, and suppression subtractive hybridization are power­ful tools for gene expression research. However, currently, the difficulty of isolating high-quality RNAs from plant tissues bearing abundant complex polysaccharides, polyphenolics, and secondary metabolites is a serious problem that not only limits the application of these technologies but also hinders studies dealing with RNA in general. We have developed a consis­tent protocol to prepare highly intact and pure RNAs from tissues of a vari­ety of field-grown plant species, with high yields, in 2 to 3 h. Additionally, this method can be readily applied to mammalian, yeast, and bacterial cells.

A recently developed revolutionary approach to transcrip­tomics, RNA-Seq, and suppression subtractive hybridization are power­ful tools for gene expression research. However, currently, the difficulty of isolating high-quality RNAs from plant tissues bearing abundant complex polysaccharides, polyphenolics, and secondary metabolites is a serious problem that not only limits the application of these technologies but also hinders studies dealing with RNA in general. We have developed a consis­tent protocol to prepare highly intact and pure RNAs from tissues of a vari­ety of field-grown plant species, with high yields, in 2 to 3 h. Additionally, this method can be readily applied to mammalian, yeast, and bacterial cells.