Research Article

Gene expression study related with the intrinsic pathway of apoptosis in bladder cancer by real-time PCR technique

Published: April 02, 2013
Genet. Mol. Res. 12 (2) : 878-886 DOI: https://doi.org/10.4238/2013.April.2.4
Cite this Article:
D.F. Barione, F.S.N. Lizarte, P.C. Novais, C.A.M. de Carvalho, F.C.B. Valeri, F.M. Peria, H.F. de Oliveira, D.L. Zanette, W.A. Silva, A.J. Cologna, R.B. Reis, S. Tucci, A.C.P. Martins, D.P.C. Tirapelli, L.F. Tirapelli (2013). Gene expression study related with the intrinsic pathway of apoptosis in bladder cancer by real-time PCR technique. Genet. Mol. Res. 12(2): 878-886. https://doi.org/10.4238/2013.April.2.4
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Abstract

We examined the expression of anti-apoptotic genes (XIAP and Bcl-2) and apoptotic genes (cytochrome c, caspase-9, Apaf-1) in tissue samples of patients with superficial bladder cancer. Thirty-two bladder cancer tissue samples (8 papillary urothelial neoplasm of low malignant potential, 10 low-grade, and 14 high-grade) and 8 normal bladder tissue samples from necropsy were used for the study of gene expression by real-time PCR analysis. Analysis of the expression of apoptotic gene constituents of an apoptosome demonstrated an increase in Apaf-1 expression in the three tumor grades when compared with the control (P caspase-9 in all groups (P cytochrome c expression in all tumor grades in relation to the control, although without statistically significant difference. The expression of anti-apoptotic genes revealed an increase in XIAP expression in all tumor grades in relation to the control, although without statistically significant difference, and low expression of Bcl-2 in all tumor grades and the control (P caspase-9 and considerable increase in XIAP expression, which may render these genes potential therapeutic targets in bladder cancer treatment.

We examined the expression of anti-apoptotic genes (XIAP and Bcl-2) and apoptotic genes (cytochrome c, caspase-9, Apaf-1) in tissue samples of patients with superficial bladder cancer. Thirty-two bladder cancer tissue samples (8 papillary urothelial neoplasm of low malignant potential, 10 low-grade, and 14 high-grade) and 8 normal bladder tissue samples from necropsy were used for the study of gene expression by real-time PCR analysis. Analysis of the expression of apoptotic gene constituents of an apoptosome demonstrated an increase in Apaf-1 expression in the three tumor grades when compared with the control (P caspase-9 in all groups (P cytochrome c expression in all tumor grades in relation to the control, although without statistically significant difference. The expression of anti-apoptotic genes revealed an increase in XIAP expression in all tumor grades in relation to the control, although without statistically significant difference, and low expression of Bcl-2 in all tumor grades and the control (P caspase-9 and considerable increase in XIAP expression, which may render these genes potential therapeutic targets in bladder cancer treatment.