Research Article

Differential expression of lipid metabolism genes in the liver and adipose tissue of mice treated with evodiamine

Published: May 13, 2013
Genet. Mol. Res. 12 (2) : 1501-1510 DOI: 10.4238/2013.January.4.13

Abstract

Evodiamine is the main active alkali of Wu Zhuyu, a widely distributed Chinese herb. It plays an important role in the regulation of body fat deposition. In this study, we aimed to investigate the effect of evodiamine administration on the expression of genes involved in lipid metabolism in the liver and adipose tissue. Fasted mice were subcutaneously injected with evodiamine (37°C, 20 mg/kg), and the core body temperature change and expression levels of lipid metabolism-related genes were evaluated at baseline, 0.5, 1, and 2 h. We detected the mRNA expression of fatty acid synthesis enzyme (FAS), peroxisome proliferator-activated receptor gamma (PPAR-γ), sterol regulatory element binding protein 1c (SREBP-1c), triglyceride hydrolase (TGH), and hormone-sensitive lipase (HSL) by real-time PCR and analyzed their correlation with core body temperature. Our results showed that the core body temperature was reduced greater than 1°C with evodiamine treatment at 1 and 2 h (P SREBP-1c, HSL, and TGH mRNA expression was significantly increased, and they reached the highest levels 1 h after injection (P PPAR-γmRNA expression was decreased and reached a significant level at 0.5 h (P FAS mRNA expression was not significantly different; FAS and SREBP-1c mRNA expression were reduced and reached significant levels at 1 h (P HSL mRNA expression was significantly reduced at 0.5 h (P TGH, HSL, FAS, and SREBP- 1c mRNA in the liver (P FAS and SREBP-1c mRNA in adipose tissue (P

Evodiamine is the main active alkali of Wu Zhuyu, a widely distributed Chinese herb. It plays an important role in the regulation of body fat deposition. In this study, we aimed to investigate the effect of evodiamine administration on the expression of genes involved in lipid metabolism in the liver and adipose tissue. Fasted mice were subcutaneously injected with evodiamine (37°C, 20 mg/kg), and the core body temperature change and expression levels of lipid metabolism-related genes were evaluated at baseline, 0.5, 1, and 2 h. We detected the mRNA expression of fatty acid synthesis enzyme (FAS), peroxisome proliferator-activated receptor gamma (PPAR-γ), sterol regulatory element binding protein 1c (SREBP-1c), triglyceride hydrolase (TGH), and hormone-sensitive lipase (HSL) by real-time PCR and analyzed their correlation with core body temperature. Our results showed that the core body temperature was reduced greater than 1°C with evodiamine treatment at 1 and 2 h (P SREBP-1c, HSL, and TGH mRNA expression was significantly increased, and they reached the highest levels 1 h after injection (P PPAR-γmRNA expression was decreased and reached a significant level at 0.5 h (P FAS mRNA expression was not significantly different; FAS and SREBP-1c mRNA expression were reduced and reached significant levels at 1 h (P HSL mRNA expression was significantly reduced at 0.5 h (P TGH, HSL, FAS, and SREBP- 1c mRNA in the liver (P FAS and SREBP-1c mRNA in adipose tissue (P