Research Article

Cloning and polymorphism analysis of the 2-methyl-6-phytyl-1,4-benzoquinol methyltransferase gene (VTE3) in Arachis hypogaea, A. duranensis, and A. ipaënsis

Published: June 11, 2013
Genet. Mol. Res. 12 (2) : 1859-1871 DOI: https://doi.org/10.4238/2013.January.4.5
Cite this Article:
(2013). Cloning and polymorphism analysis of the 2-methyl-6-phytyl-1,4-benzoquinol methyltransferase gene (VTE3) in Arachis hypogaea, A. duranensis, and A. ipaënsis. Genet. Mol. Res. 12(2): gmr1982. https://doi.org/10.4238/2013.January.4.5
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Abstract

One of the critical enzymes involved in vitamin E bio­synthesis in plants is 2-methyl-6-phytyl-1,4-benzoquinol methyltrans­ferase (MPBQ MT). The full-length VTE3 cDNA (designated rVTE3-1 and -2) encoding MPBQ MT and the full-length DNA of VTE3 (des­ignated gVTE3-1 and -2) were isolated from cultivated peanuts (Ara­chis hypogaea). The full-length DNA of VTE3 (designated gVTE3-A and -B) was isolated from the wild groundnut species A. duranensis (A-genome) and A. ipaënsis (B-genome), and polymorphism analysis of VTE3 was performed. The results demonstrated that rVTE3-1 and -2 both have a DNA sequence that is 1059 bp long and encodes 351 amino acids; the homology of the 2 amino acid sequences was 98.6%. The gVTE3-1 of cultivated Fenghua 2 peanut samples was 2710 bp long, with 3 introns located at 44-163, 772-1295, and 1603-2437 bp, and the Fenghua 2 gVTE3-2 was 2706 bp long, with 3 introns located at 44-169, 778-1291, and 1599-2433 bp. The homology for gVTE3-1 and -2 across 13 cultivated peanut samples was 99.9 and 100%, respec­tively. gVTE3-1 and -2 were from A- and B-genome, respectively, with 96.6% homology between the 2 sequences. The present study demon­strated that abundant polymorphisms were present in the VTE3 genes from different genomes. Additionally, polymorphisms were observed in the gVTE3-1 alleles of the 13 cultivars and wild A. duranensis spe­cies but not in the gVTE3-2 alleles of the 13 cultivars and wild A. ipaënsis species.

One of the critical enzymes involved in vitamin E bio­synthesis in plants is 2-methyl-6-phytyl-1,4-benzoquinol methyltrans­ferase (MPBQ MT). The full-length VTE3 cDNA (designated rVTE3-1 and -2) encoding MPBQ MT and the full-length DNA of VTE3 (des­ignated gVTE3-1 and -2) were isolated from cultivated peanuts (Ara­chis hypogaea). The full-length DNA of VTE3 (designated gVTE3-A and -B) was isolated from the wild groundnut species A. duranensis (A-genome) and A. ipaënsis (B-genome), and polymorphism analysis of VTE3 was performed. The results demonstrated that rVTE3-1 and -2 both have a DNA sequence that is 1059 bp long and encodes 351 amino acids; the homology of the 2 amino acid sequences was 98.6%. The gVTE3-1 of cultivated Fenghua 2 peanut samples was 2710 bp long, with 3 introns located at 44-163, 772-1295, and 1603-2437 bp, and the Fenghua 2 gVTE3-2 was 2706 bp long, with 3 introns located at 44-169, 778-1291, and 1599-2433 bp. The homology for gVTE3-1 and -2 across 13 cultivated peanut samples was 99.9 and 100%, respec­tively. gVTE3-1 and -2 were from A- and B-genome, respectively, with 96.6% homology between the 2 sequences. The present study demon­strated that abundant polymorphisms were present in the VTE3 genes from different genomes. Additionally, polymorphisms were observed in the gVTE3-1 alleles of the 13 cultivars and wild A. duranensis spe­cies but not in the gVTE3-2 alleles of the 13 cultivars and wild A. ipaënsis species.

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