Research Article

Isolation and characterization of 16 microsatellite loci in marble goby (Oxyeleotris marmoratus)

Published: June 20, 2013
Genet. Mol. Res. 12 (2) : 2020-2023 DOI: https://doi.org/10.4238/2013.January.4.2
Cite this Article:
(2013). Isolation and characterization of 16 microsatellite loci in marble goby (Oxyeleotris marmoratus). Genet. Mol. Res. 12(2): gmr1873. https://doi.org/10.4238/2013.January.4.2
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Abstract

A microsatellite-enriched genomic library for marble goby (Oxyeleotris marmoratus), a freshwater fish of considerable economic value, was obtained. A total of 16 microsatellite markers were successfully isolated and characterized in a population from the Mekong River in Vietnam. These markers had 2-20 alleles with expected heterozygosity ranging from 0.0370 to 0.8927. Linkage equilibrium was observed in most loci, and only 1 locus revealed a significant deviation from Hardy-Weinberg equilibrium. These microsatellite markers will be useful for genetic diversity and molecular marker-assisted selection studies of wild and farmed O. marmoratus.

A microsatellite-enriched genomic library for marble goby (Oxyeleotris marmoratus), a freshwater fish of considerable economic value, was obtained. A total of 16 microsatellite markers were successfully isolated and characterized in a population from the Mekong River in Vietnam. These markers had 2-20 alleles with expected heterozygosity ranging from 0.0370 to 0.8927. Linkage equilibrium was observed in most loci, and only 1 locus revealed a significant deviation from Hardy-Weinberg equilibrium. These microsatellite markers will be useful for genetic diversity and molecular marker-assisted selection studies of wild and farmed O. marmoratus.

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