Research Article

Effects of various salinities on Na+-K+-ATPase, Hsp70 and Hsp90 expression profiles in juvenile mitten crabs, Eriocheir sinensis

Published: April 19, 2012
Genet. Mol. Res. 11 (2) : 978-986 DOI: 10.4238/2012.April.19.3

Abstract

Eriocheir sinensis is a euryhaline crab migrating from sea to freshwater habitats during the juvenile stage. We used quantitative real-time polymerase chain reaction (qRT-PCR) to investigate the gene expression profile of Na+-K+-ATPase, Hsp70 (heat shock protein 70) and Hsp90 in megalopa exposed to salinities of 0, 2, 5, 10, and 15 parts per thousand. Both low and high salinities markedly stimulated expression of Na+-K+-ATPase, Hsp70 and Hsp90 genes of Chinese mitten crab megalopa; salinity had different effects on Na+-K+-ATPase, Hsp70 and Hsp90 levels depending on the duration of salinity stress, implying that Na+-K+-ATPase, Hsp70 and Hsp90 may play an important role in salinity tolerance in this crab species.

Eriocheir sinensis is a euryhaline crab migrating from sea to freshwater habitats during the juvenile stage. We used quantitative real-time polymerase chain reaction (qRT-PCR) to investigate the gene expression profile of Na+-K+-ATPase, Hsp70 (heat shock protein 70) and Hsp90 in megalopa exposed to salinities of 0, 2, 5, 10, and 15 parts per thousand. Both low and high salinities markedly stimulated expression of Na+-K+-ATPase, Hsp70 and Hsp90 genes of Chinese mitten crab megalopa; salinity had different effects on Na+-K+-ATPase, Hsp70 and Hsp90 levels depending on the duration of salinity stress, implying that Na+-K+-ATPase, Hsp70 and Hsp90 may play an important role in salinity tolerance in this crab species.