Research Article

A novel SNP of the C/EBPα gene associated with superior meat quality in indigenous Chinese cattle

Published: September 16, 2011
Genet. Mol. Res. 10 (3) : 2069-2077 DOI: https://doi.org/10.4238/vol10-3gmr1032
Cite this Article:
(2011). A novel SNP of the C/EBPα gene associated with superior meat quality in indigenous Chinese cattle. Genet. Mol. Res. 10(3): gmr1032. https://doi.org/10.4238/vol10-3gmr1032
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Abstract

CCAAT/enhancer-binding protein alpha (C/EBPa) is an essential transcriptional factor regulating the differentiation of adipocytes. We report a novel single nucleotide polymorphism (C271A) of the C/EBPa gene in six indigenous Chinese cattle breeds using PCR-SSCP and DNA sequencing methods. Allele frequencies were investigated and evaluated by the χ2 test in 817 individuals; all populations were found to be in Hardy-Weinberg equilibrium. Gene heterozygosity, effective allele numbers and polymorphism information content of the C/EBPa locus varied from 0.50 to 0.54, 1.84 to 1.99 and 0.35 to 0.37, respectively. We also evaluated a potential association of the C/EBPa SNP with ultrasound traits in 555 individuals; individuals of the AA genotype had greater ultrasound backfat thickness than did genotype CC (0.36 versus 0.34 cm, P 2, P

CCAAT/enhancer-binding protein alpha (C/EBPa) is an essential transcriptional factor regulating the differentiation of adipocytes. We report a novel single nucleotide polymorphism (C271A) of the C/EBPa gene in six indigenous Chinese cattle breeds using PCR-SSCP and DNA sequencing methods. Allele frequencies were investigated and evaluated by the χ2 test in 817 individuals; all populations were found to be in Hardy-Weinberg equilibrium. Gene heterozygosity, effective allele numbers and polymorphism information content of the C/EBPa locus varied from 0.50 to 0.54, 1.84 to 1.99 and 0.35 to 0.37, respectively. We also evaluated a potential association of the C/EBPa SNP with ultrasound traits in 555 individuals; individuals of the AA genotype had greater ultrasound backfat thickness than did genotype CC (0.36 versus 0.34 cm, P 2, P

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