Research Article

Single nucleotide polymorphism analysis of exons 3 and 4 of IGF-1 gene in pigs

Published: August 16, 2011
Genet. Mol. Res. 10 (3) : 1689-1695 DOI: https://doi.org/10.4238/vol10-3gmr1328
Cite this Article:
L.L. Hao, H. Yu, Y. Zhang, S.C. Sun, S.C. Liu, Y.Z. Zeng, Y.X. Ai, H.Z. Jiang (2011). Single nucleotide polymorphism analysis of exons 3 and 4 of IGF-1 gene in pigs. Genet. Mol. Res. 10(3): 1689-1695. https://doi.org/10.4238/vol10-3gmr1328
2,170 views

Abstract

The IGF-1 gene has been implicated as a candidate gene for the regulation of pig growth traits. We analyzed exons 3 and 4 of IGF-1 gene polymorphisms of the Banna mini-pig (28), the Tibetan mini-pig (30), the Junmu pig (55), and L. Yorkshire species (50) using PCR-SSCP. Three genotypes in exon 3 and 6 genotypes in exon 4 were observed, among which, one single nucleotide polymorphism, G201A, on exon 3 and two single nucleotide polymorphisms, A440G and T455C, on exon 4 were found. Statistical analysis of genotype frequencies revealed that the A allele was dominant in the large pig at the G201A locus (PIC = 0.20-0.34), and the AT alleles were dominant in the large pig at the A440G and T455C loci (PIC = 0.30-0.60). The genotype distribution between the various groups was significantly different (P

The IGF-1 gene has been implicated as a candidate gene for the regulation of pig growth traits. We analyzed exons 3 and 4 of IGF-1 gene polymorphisms of the Banna mini-pig (28), the Tibetan mini-pig (30), the Junmu pig (55), and L. Yorkshire species (50) using PCR-SSCP. Three genotypes in exon 3 and 6 genotypes in exon 4 were observed, among which, one single nucleotide polymorphism, G201A, on exon 3 and two single nucleotide polymorphisms, A440G and T455C, on exon 4 were found. Statistical analysis of genotype frequencies revealed that the A allele was dominant in the large pig at the G201A locus (PIC = 0.20-0.34), and the AT alleles were dominant in the large pig at the A440G and T455C loci (PIC = 0.30-0.60). The genotype distribution between the various groups was significantly different (P

Key words: