Research Article

Genetic relationships among wild pomegranate (Punica granatum genotypes from Coruh Valley in Turkey

Published: March 15, 2011
Genet. Mol. Res. 10 (1) : 459-464 DOI: https://doi.org/10.4238/vol10-1gmr1155
Cite this Article:
(2011). Genetic relationships among wild pomegranate (Punica granatum genotypes from Coruh Valley in Turkey. Genet. Mol. Res. 10(1): gmr1155. https://doi.org/10.4238/vol10-1gmr1155
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Abstract

The pomegranate has been used traditionally in Coruh Valley in Turkey for a long time; fruits are harvested from wild, semi-domesticated and cultivated trees. In the valley, it oc­curs in general along with olive trees. We sampled 23 wild-grown pomegranate genotypes sampled from different parts of Coruh Valley and compared them using RAPD primers to determine ge­netic variability. Eighty-six RAPD primers were used for molecu­lar characterizations, among which 12 gave reliable polymorphic patterns. These primers generated 145 RAPD bands of which 91% were polymorphic. The highest polymorphism ratio was observed with primers OPY06, OPY13, OPBA03, OPBB03, OPBB07, and OPBB09 (100%), while the lowest was with OPBB09 and OPBB10 (75%). The band size was between 250 and 2400 bp. There were five main clusters in the dendrogram; the highest genetic similarity was 0.24 and the lowest was 0.08.

The pomegranate has been used traditionally in Coruh Valley in Turkey for a long time; fruits are harvested from wild, semi-domesticated and cultivated trees. In the valley, it oc­curs in general along with olive trees. We sampled 23 wild-grown pomegranate genotypes sampled from different parts of Coruh Valley and compared them using RAPD primers to determine ge­netic variability. Eighty-six RAPD primers were used for molecu­lar characterizations, among which 12 gave reliable polymorphic patterns. These primers generated 145 RAPD bands of which 91% were polymorphic. The highest polymorphism ratio was observed with primers OPY06, OPY13, OPBA03, OPBB03, OPBB07, and OPBB09 (100%), while the lowest was with OPBB09 and OPBB10 (75%). The band size was between 250 and 2400 bp. There were five main clusters in the dendrogram; the highest genetic similarity was 0.24 and the lowest was 0.08.