Research Article

First morphogenetic identification of the fungal pathogen Colletotrichum musae (Phyllachoraceae) from imported bananas in Saudi Arabia

Published: November 30, 2010
Genet. Mol. Res. 9 (4) : 2335-2342 DOI: https://doi.org/10.4238/vol9-4gmr972
Cite this Article:
(2010). First morphogenetic identification of the fungal pathogen Colletotrichum musae (Phyllachoraceae) from imported bananas in Saudi Arabia. Genet. Mol. Res. 9(4): gmr972. https://doi.org/10.4238/vol9-4gmr972
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Abstract

Colletotrichum musae is the causal agent of anthrac­nose in banana fruits; infection by this fungal pathogen results in severe post-harvest losses. Eleven C. musae isolates were obtained from infected imported banana fruit samples with anthracnose le­sions collected from different markets in Riyadh, Saudi Arabia. The pathogenic, morphological, cultural, and molecular characteristics of these C. musae isolates were evaluated. The cultures had char­acteristic fast-growing sparse aerial mycelia that were white, with copious cinnamon conidial masses, conidia usually elliptical, and setae absent. An inoculation test was used to determine whether isolates could cause anthracnose symptoms on banana fruits. Ne­crotic lesions developed and orange-colored spore structures were later observed on these lesions. Microsatellite-primed PCR (MP-PCR) was used to identify genetic variation among the C. musae isolates. The dendrogram obtained from cluster analysis of the MP-PCR fingerprints revealed a great deal of homogeneity among the isolates, shown by the formation of two clusters. Intraspecific simi­larity among the C. musae isolates ranged from 83 to 100%. This is the first report demonstrating morphological and genetic variation within a population of C. musae in Saudi Arabia.

Colletotrichum musae is the causal agent of anthrac­nose in banana fruits; infection by this fungal pathogen results in severe post-harvest losses. Eleven C. musae isolates were obtained from infected imported banana fruit samples with anthracnose le­sions collected from different markets in Riyadh, Saudi Arabia. The pathogenic, morphological, cultural, and molecular characteristics of these C. musae isolates were evaluated. The cultures had char­acteristic fast-growing sparse aerial mycelia that were white, with copious cinnamon conidial masses, conidia usually elliptical, and setae absent. An inoculation test was used to determine whether isolates could cause anthracnose symptoms on banana fruits. Ne­crotic lesions developed and orange-colored spore structures were later observed on these lesions. Microsatellite-primed PCR (MP-PCR) was used to identify genetic variation among the C. musae isolates. The dendrogram obtained from cluster analysis of the MP-PCR fingerprints revealed a great deal of homogeneity among the isolates, shown by the formation of two clusters. Intraspecific simi­larity among the C. musae isolates ranged from 83 to 100%. This is the first report demonstrating morphological and genetic variation within a population of C. musae in Saudi Arabia.